Table 1.

Antibody Design and Reactivity

Antibody	Designed to Recognize	Peptide Sequence	Reactivity to Denatured IMPDH1	Reactivity to Denatured IMPDH2	Reactivity In Vivo
Anti-N-IMPDH1	Canonical IMPDH1 residues 38–50	GFIDFIADEVDLT	++++	—	—
Anti-N-IMPDH2	IMPDH2 residues 38–50	GYIDFTADQVDLT	—	+++	—
Anti-C-IMPDH1	Canonical IMPDH1 residues 426–438	SQKRYFSEGDKVK	+++++	+	+++
Anti-C-IMPDH1+2	IMPDH2 residues 426–438	SQNRYFSEADKIK	++++	++++>	+++
Anti-exon A-IMPDH1	IMPDH1 retinal isoform	EPGARQHPGHETAAQRYSAR	+++++ Exon A isoform	—	ND
Anti-exon 13b-IMPDH1	IMPDH1 retinal isoform	YTFLPFTRSGCTED	++ Exon 13b isoform	—	ND

ND, not determined; + indicates binding affinity.