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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: J Immunol. 2008 Oct 15;181(8):5681–5690. doi: 10.4049/jimmunol.181.8.5681

FIGURE 2.

FIGURE 2

Histology of CNS inflammation and demyelination in Stat4α and Stat4β transgenic mice with EAE. The spinal cord samples were isolated from C57BL/6 wild type (WT), Stat4 deficient (Stat4−/−), Stat4α transgenic (Stat4α) and Stat4β transgenic (Stat4β) mice on day 30 following induction of EAE. The transverse sections of cervical, upper thoracic, lower thoracic and lumbar regions of spinal cord were obtained and stained with LFB/PAS (luxol fast blue/periodic acid scriff) along with H&E (hematoxylin and eosin). The pathology of demyelination (left) and inflammation (right) in the spinal cord sections were visualized by microscopy and the representative 10X pictures are shown. The number of positive quadrants with inflammation and demyelination were scored and expressed as percentage over the total number of quadrants examined in the histogram.