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Lagging Strand Unwinding Catalyzed by Fml1ΔC
(A–C) Unwinding assays showing Fml1ΔC's ability to dissociate fork substrates F2, F10 and F11. Schematics of the substrates and reaction products are shown with asterisks indicating the 32P label at the DNA 5′ end. (D and F) Time courses of F11 and F10 unwinding. Reactions (100 μl) contain Fml1ΔC (2 nM) and 32P labeled F11 or F10 (0.5 nM) as indicated. (E) Quantification of data in (D). (G) Quantification of data in (F).
