Figure 5. IL-6 attracts EPC migration.
A. Bar graph shows that IL-6-stimulated EPCs to migrate across a porous filter judged by Boyden chamber assays. IL-6 induced EPC migration in a dose-dependent manner. B. Bar graph shows that IL-6 significantly increased EPC migration compared to the negative control. This action was attenuated using IL-6 antibody (50 ng/ml) neutralizing or IL-6 receptor antibody (2 μg/ml) inhibition. VEGF (20 ng/ml) acted as a positive control and PBS as a negative control. Each experiment was performed three times. The results are mean±SD, n=3, *=p<0.01, IL-6 treated group vs. the control; #=p<0.05, IL-6 antibody treated group vs. IL-6 plus antibody treated or IL-6 receptor treated groups.