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Stopped-flow fluorescence traces obtained upon mixing (Aα2-L1M/L38M)2 with either 2.85 mM sevoflurane in buffer or buffer alone. The same fluorescence traces are shown in panels A & B on different timescales. Trace amplitudes were normalized between 0 (buffer only) and 1 (protein, no anesthetic). The curve is a fit of the decay to a double exponential equation to obtain the normalized amplitudes and rates of the fast and slow components.
