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. Author manuscript; available in PMC: 2009 Nov 7.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Sep 5;376(1):200–205. doi: 10.1016/j.bbrc.2008.08.124

Figure 1.

Figure 1

(A) Diagrammatic depiction of the effect of a mutant β-globin intron-2 (IVS2-654) insert in the luciferase gene coding sequence on luciferase expression. Blockade of the aberrant IVS2-654 splice site by an antisense oligomer (right-hand diagram) permits expression of a correctly spliced gene product and active enzyme. Adapted from Kang et al. [25] and Sazani et al. [10]. (B) Induction of luciferase expression and (C) correction of pre-mRNA splicing in Luc-IVS2-654 CHO cells following 48 hrs incubation with 1.0µM antisense (AS) PNA(Lys)8 oligomers delivered to the cells by cell scraping transfection [16] and oligofectamine. Results in B represent mean values ± SEM for 4 replicate studies.