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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Dev Cell. 2008 Aug;15(2):248–260. doi: 10.1016/j.devcel.2008.06.013

Figure 1. CV2 is a Secreted BMP Feedback Inhibitor.

Figure 1

(A) Normal CV2 expression at stage 22 (hemisection). vb, ventral blastopore

(B) CV2MO microinjection increases CV2 expression.

(C) The CV2 negative feedback loop requires BMP4. For each experimental sample at least 25 embryos were examined, with similar results.

(D) qRT-PCR showing increased expression of the ventral marker Vent1 in CV2-depleted embryos.

(E) CV2 depletion reduces expression of the dorsal/forebrain marker Six3.

(F) Endogenous Smad1 phosphorylation is increased by CV2 depletion at gastrula and neurula stages 12, 13 and 14. A pSmad1 signal was detectable in the stage 14 uninjected lane upon longer exposure. Total Smad1 antibody (T-Smad1) staining was used as loading control.

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