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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Dev Cell. 2008 Aug;15(2):248–260. doi: 10.1016/j.devcel.2008.06.013

Figure 4. CV2, BMP4 and Tsg form a Ternary Complex that Inhibits BMP Signaling.

Figure 4

(A) DSS crosslinking showing that CV2 binds BMP4 directly.

(B) CV2 dose-dependently blocks BMP4-induced phosphorylation of endogenous Smad1 in mouse L-cells; BMP4 was added for 30 min in serum-free medium after pre-incubation with CV2 protein for 2 hours at 4°C.

(C) Co-immunoprecipitation demonstrating that purified CV2 binds Tsg.

(D) Tsg facilitates the binding of CV2 to BMP4.

(E) CV2, BMP4 and Tsg form a ternary complex. Left panel shows DSS crosslinking products stained with anti-CV2 antibody. Right panel, same samples examined with anti-BMP monoclonal antibody (anti-Tsg antibody showed smears due to crosslinking of Tsg with itself). Tsg protein alone is not recognized by either antibody (not shown).

(F) CV2 and Tsg additively block the binding of BMP4 to BMPR-1b-Fc.

(G) Model of the molecular interactions of CV2, Tsg and BMP4 in a ternary complex.

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