Table 1.
Effects of 9-cis-RA on the Gene Expression in Ca9-22 Cells.
| Up-Regulated Genes | GenBank No. | Fold Intensitya | Expression Typeb | Primer pairs |
|---|---|---|---|---|
| Solute carrier family 37 (glycerol-3-phosphate transporter), member 2 (SLC37A2) | XM_166184.1 | 4.23±0.23 | B | AGCCTCACCCTGCTCTCCTTCC / CGGGCCTGCTTTTCTGCTTAAT |
| Ankyrin repeat and SOCS box-containing 2 (ASB2) | NM_016150.3 | 4.12±0.23 | A | CGCCCAGAGTGGACAGTTGGAG / GGCACCCTGTGACAGCAGAAAC |
| Prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) (PSAP) | NM_002778.1 | 4.05±0.12 | C | GAATGTGAAGACGGCGTCCGACT / CTCCTCAGTGGCATTGTCCTTCAG |
| Similar to cytokeratin (AA 1-513) (LOC144501) | XM_096612.7 | 3.90±0.19 | B | AAAGCAGCTTGGAGCCTCTAGGT / GATACTGATATGGAGCGGAGTGGC |
| Tenascin C (hexabrachion) (TNC) | NM_002160.1 | 3.68±1.41 | A | CATGACTCAGCTGTTGGCAGGT / AAACGTGGTTAAACACCACTGGC |
| Transmembrane protease, serine 4 (TMPRSS4) | NM_019894.1 | 3.42±0.29 | C | ATTGACAGCACACGGTGCAATG / CCACATGCCACTGGTCAGATTGG |
| Kallikrein 6 (neurosin, zyme) (KLK6) | NM_002774.2 | 3.29±0.04 | A | GAGGGACCTACGGGCAGCTGTT / CCATGCACCAACTTATTCTGCTCC |
| Sphingosine kinase 1 (SPHK1) | NM_021972.2 | 3.03±0.32 | A | AGGAAGAGTGGGTTCCAAGACACC / GAGTGCAGCAGTGCCAGGACTAGC |
| Carbonyl reductase 3 (CBR3) | NM_001236.2 | 2.85±0.85 | C | AACATGTGCAACGAGTTACTGCCG / CCACCAGGTCTCCTTCTGTGAGTG |
| Hypothetical protein FLJ25348 (FLJ25348) | NM_144569.2 | 2.78±0.13 | B | TCAAGTCTGTTGCTGGCTGTGCT / CATTCGGCCTCCTGTCATCTGG |
| Midkine (neurite growth-promoting factor 2) (MDK) | NM_002391.2 | 2.66±0.11 | C | AGTGCCTTCTGTCTGCTCGTTAGC / GGCTCTGGGACTCACATTGCTTT |
| Matrix metalloproteinase 12 (macrophage elastase) (MMP12) | NM_002426.1 | 2.57±0.14 | A | GGGCCCGTATGGAGGAAACATTAT / CCATGCCTGTGTTAATCTTGCTGA |
| Chromosome 20 open reading frame 108 (C20orf108) | NM_080821.1 | 2.46±0.66 | C | CTGCCCTGTCACGTAGTTGAAGG / CCAGGATGGCCAGTTAATGGTTT |
| LOC158819 (LOC158819) | XM_098995.1 | 2.42±0.04 | A | TGCCATCTTCTCAGTCCTTCTCC / GACCCAGTAGT AGGCACTGACGGT |
| Down-Regulated Genes | ||||
| Homo sapiens heparin-binding growth factor binding protein (HBP17), | NM_005130.1 | 0.08±0.01 | NA | AGCCTCACCCTGCTCTCCTTCC / CGGGCCTGCTTTTCTGCTTAAT |
| Troponin T3, skeletal, fast (TNNT3) | NM_006757.1 | 0.13±0.05 | NA | AAGGCCAAGGAGCTCTGGGAGA / CTGTGCTTCTGGGCCTGGTCAAT |
| Dickkopf homolog 1 (Xenopus laevis) (DKK1) | NM_012242.1 | 0.22±0.001 | NA | CCGAGGAGAAATTGAGGAAACCAT / GGCACAGTCTGATGACCGGAGA |
| ATPase, Na+/K+ transporting, beta 1 polypeptide (ATP1B1) | NM_001677.1 | 0.22±0.03 | NA | TCTTGCCTTGTCCTCCGGTATGT / CAGCACCAGCATGTGATGCCTC |
| Keratin 6C (KRT6C) | NM_058242.1 | 0.25±0.03 | NA | AGCGTGAGCAGATCAAGACCCTC / ACAACGGCTCCAGGTTCTGCCT |
| Keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) (KRT5) | NM_000424.2 | 0.30±0.07 | NA | GAGGCCAAGGTTGATGCACTGAT / CGATGATGCTATCCAGGTCCAGG |
| CD44 antigen (homing function and Indian blood group system) (CD44) | NM_000610.2 | 0.34±0.02 | NA | GGCTGATCATCTTGGCATCCCT / TTGAGTCCACTTGGCTTTCTGTCC |
| Dehydrogenase/reductase (SDR family) member 2 (DHRS2) | NM_005794.1 | 0.36±0.14 | NA | TAGAACACTGGCATTGGAGCTGGC / CCCAATCCTCTGCAGCTGATGA |
| Immediate early response 5 (IER5) | NM_016545.2 | 0.37±0.03 | NA | ACTTTACACCTACCCCTCACCGGA / TCTTCATTGGGAGAGAAAGGTGGG |
| Serum/glucocorticoid regulated kinase (SGK) | NM_005627.2 | 0.39±0.01 | NA | ATTATGTCGGAGCGGAATGTTCTG / AGCGTTCCCTCTGGAGATGGTAGA |
| Homo sapiens serpin peptidase inhibitor, clade B (ovalbumin), member 5(SERPINB5) | NM_002639 | 0.39±0.07 | NA | TGAACGACCAGACCAAAATCCTTG / CGTGGCCTCCATGTTCATCATCT |
| Angiomotin like 2 (AMOTL2) | XM_300852.1 | 0.40±0.10 | NA | AGAAGGATGCAGTGATCAAGGTCC / TCACTAGAAGAGAGCCCTTGCCA |
| Matrix metalloproteinase 1 (interstitial collagenase) (MMP1) | NM_002421.2 | 0.42±0.01 | NA | GATGGACCTGGAGGAAATCTTGCT / CAAGAGAATGGCCGAGTTCATGA |
| Plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1) | NM_000299.1 | 0.43±0.05 | NA | TTACAGCACCTGCAGTGGTCAGAA / GCAGATCTTGGAGCTGGCCCTAG |
| Caveolin 1, caveolae protein, 22kDa (CAV1) | NM_001753.3 | 0.47±0.04 | NA | CCCTTAAAGCACAGCCCAGGGA / GGCCTTGTTGTTGGGCTTGTAGA |
| ATPase, Na+/K+ transporting, beta 3 polypeptide (ATP1B3) | NM_001679.1 | 0.49±0.17 | NA | AGCCTGAAGGAGTGCCAAGGATA / GCAACCAATGGCTGTAGATACCCA |
| Keratin 19 (KRT19) | NM_002276.2 | 0.51±0.07 | NA | GCCAGGTCAGTGTGGAGGTGGAT / TCCCGGTTCAATTCTTCAGTCCG |