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. 2008 Nov 14;283(46):31960–31967. doi: 10.1074/jbc.M805431200

FIGURE 7.

FIGURE 7.

LRP130 induces Ucp1 and is enriched on the Ucp1 promoter. A, stable expression of exogenous human LRP130-FLAG in mature 3T3-F442A cells (n = 3). B, expression of brown fat genes in mature 3T3-F442A cells, stably expressing vector or human LRP130-FLAG (n = 3). C, luciferase reporter assay using the -4kbto 1+ bp Ucp1 promoter in mature 3T3-F442A cells. Measurements were performed on days 2, 4, and 8 of differentiation. RLU denotes relative luciferase units (n = 3). D, chromatin immunoprecipitation of LRP130 in mature brown fat cells. Q-PCR was used to quantify enrichment at the -4 kb PPRE of the aP2 promoter (-4kb aP2), + 1 to -200 bp region of the Ucp1 promoter (Ucp1 p), -2500 to -2300 bp region of the Ucp1 enhancer(Ucp1 e), and a random control site in the 18S gene. Error bars represent mean (±S.E.). *, p < 0.05; **, p < 0.01; ***, p < 0.001.