FIGURE 2.

Coexpression of Clock, Per3, and LC opsin mRNA in red cone photoreceptor cells. A, diagram showing the principal (red cone) and associate (green cone) members of double cone photoreceptor cells. B, C, images that show the morphology of double cone cells and immunoreactivity of red cone opsin antibodies in the outer segment of red cone cell. Scale bar, 30 μm. D, single-cell RT-PCR showing the expression of Clock, Per3, and LC opsin in a red cone photoreceptor cell. Molecular markers are shown in the left lane of the gel. E, RT-PCR showing that LC opsin mRNA is expressed in the eye but not in embryos in which the eyes were enucleated. Actin (control) was detected in both samples.