FIGURE 2.

Effects of KAT3B on 15-LOX-1 promoter histone acetylation and 15-LOX-1 transcriptional activation. A, effects of KAT3B siRNA transfection on KAT3B mRNA expression. Caco-2 cells were transfected with a pool of four siRNA duplexes for KAT3B (KAT3B siRNA), a nonspecific siRNA sequence (Nonspecific siRNA), or transfection medium only (Control) and then treated 24 h later with either depsipeptide (5 nm) or control solvent. The cells were harvested 48 h after transfection. KAT3B mRNA levels were measured by using real-time reverse transcription-PCR. The relative expression levels were calculated as the values relative to those of the calibrator samples (Nonspecific siRNA). B and C, effects of KAT3B knockdown on H3 (B) and H4 (C) acetylation in the 15-LOX-1 promoter. Caco-2 cells were transfected and treated as in A and then subjected to chromatin immunoprecipitation/real-time PCR. The results are presented as percentages of the respective input genomic DNA for the 15-LOX-1 promoter. The values are the means ± S.D. of triplicate measurements. D, effects of KAT3B knockdown on depsipeptide activation of 15-LOX-1 transcription. Caco-2 cells were transfected and treated as described in A, and 15-LOX-1 expression was measured by using real-time reverse transcription-PCR. The relative expression levels were calculated as the values relative to those of the calibrator samples (Control). The values shown are the means ± S.D. of triplicate experiments.