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. 2008 Nov 14;283(46):31341–31347. doi: 10.1074/jbc.M803729200

FIGURE 4.

FIGURE 4.

Chromatin modification of 15-LOX-1 promoter during transcriptional activation. A, kinetics of depsipeptide effects on dimethyl-histone H3 lysine 4 (H3K4me2) in the 15-LOX-1 promoter. Caco-2 cells were treated with depsipeptide as indicated. ChIP/real-time PCR was performed with the use of specific H3K4me2 or acetylated histone H3 (acetylated H3) antibodies. The results are the means ± S.D. of four replicate experiments. B, effects of depsipeptide on H3 and H4 methylation patterns in the 15-LOX-1 promoter. Caco-2 cells were treated with depsipeptide (5 nm) or solvent control, harvested 30 min later, and processed for ChIP/real-time PCR using anti-H3K9me1, anti-H3K9me2, anti-H3K9me3, anti-H4K20me3, anti-H3K27me2, and anti-H3K27me3 antibodies. The results are the means ± S.D. of triplicate experiments. C and D, kinetics of depsipeptide effects on H3 acetylation and methylation. Caco-2 cells were treated with depsipeptide for the indicated times and processed for ChIP/real-time PCR using antibodies against H3K9me2 (C) and H3K9ac (D). The results are the means ± S.D. of four replicate experiments.