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. 2008 Nov 14;283(46):32099–32109. doi: 10.1074/jbc.M806817200

FIGURE 7.

FIGURE 7.

Alanine substitution at the PheA23 position alters the receptor-interaction activities of RLN2. A, schematic representation of RLN2 mutants with alanine substitution at the ArgA22 or PheA23 positions. B, stimulation of cAMP production in LGR7- and LGR8-expressing cells by RLN2 RA22A and RLN2 FA23A mutants. FA23A mutation ablated LGR8-activation activity. C, LGR7- and LGR8-binding activities of RA22A and FA23A mutant peptides. FA23A mutation significantly reduced LGR8-binding activity, but without an effect on LGR7-binding activity. Each data point in the receptor-activation and receptor-binding assays represents the mean ± S.E. of quadruplicate and triplicate samples, respectively.