TABLE 1.
Potencies of recombinant wild type (WT) and mutant RLN2 peptides on the activation of LGR7 and LGR8
|
Ligand
|
pEC50
|
|
|---|---|---|
| LGR7 | LGR8 | |
| Nontagged RLN2 | 8.53 ± 0.10 | |
| WT RLN2 (His1) | 8.66 ± 0.09 | |
| WT RLN2 (His2) | 8.77 ± 0.10 | |
| RLN2 C-104 | 8.74 ± 0.05 | |
| RLN2 C-38 | 8.72 ± 0.10 | |
| RLN2 C-28 | 8.66 ± 0.07 | |
| RLN2 C-18 | 8.74 ± 0.09 | |
| RLN2 C-8 | 8.81 ± 0.10 | |
| WT RLN2 | 9.30 ± 0.04 | 7.41 ± 0.14 |
| WT RLN3 | 7.73 ± 0.07 | NDa |
| WT INSL3 | ND | 9.03 ± 0.05 |
| RLN2 RB12A | ND | ND |
| RLN2 RB16A | ND | ND |
| RLN2 TA16A | 9.75 ± 0.08 | 8.04 ± 0.06 |
| RLN2 KA17A | 9.18 ± 0.07 | 8.39 ± 0.06 |
| RLN2 RA18A | 9.67 ± 0.10 | 7.56 ± 0.04 |
| RLN2A19-20 | 8.56 ± 0.07 | 8.41 ± 0.07 |
| RLN2A22-23 | 8.87 ± 0.07 | ND |
| RLN2 RA22A | 10.03 ± 0.07 | 7.51 ± 0.09 |
| RLN2 FA23A | 9.45 ± 0.09 | ND |
| RLN2 KA17G | 9.04 ± 0.05 | 8.28 ± 0.05 |
| RLN2 KA17Q | 9.31 ± 0.11 | 7.77 ± 0.05 |
| RLN2 KA17D | 8.41 ± 0.06 | 8.36 ± 0.08 |
a
ND, the pEC50 values were not measurable.