TABLE 2.
Potencies of wild type (WT) and mutant RLN2 peptides in competition for RLN2 binding to LGR7 and LGR8
|
Ligand
|
pIC50
|
|
|---|---|---|
| LGR7 | LGR8 | |
| Nontagged RLN2 | 8.78 ± 0.12 | |
| RLN2 C-8 | 8.61 ± 0.09 | |
| WT RLN2 | 8.72 ± 0.06 | 7.90 ± 0.16 |
| RLN2 TA16A | 8.40 ± 0.06 | 7.74 ± 0.07 |
| RLN2 KA17A | 8.36 ± 0.08 | 8.14 ± 0.18 |
| RLN2 RA18A | 8.42 ± 0.08 | 7.46 ± 0.17 |
| RLN2A19-20 | 8.21 ± 0.10 | 7.51 ± 0.12 |
| RLN2A22-23 | 7.50 ± 0.16 | NDa |
| RLN2 RA22A | 8.42 ± 0.08 | 6.80 ± 0.32 |
| RLN2 FA23A | 8.17 ± 0.06 | ND |
| RLN2 KA17G | 7.57 ± 0.22 | 7.40 ± 0.41 |
| RLN2 KA17Q | 7.93 ± 0.15 | 7.96 ± 0.11 |
| RLN2 KA17D | 7.75 ± 0.25 | 7.53 ± 0.24 |
a
ND, the pIC50 values were not measurable.