FIGURE 5.

Neuronostatin attracts the growth cone of cerebellar granule cells. A, microscopic images of the growth cone of a cultured cerebellar granule neuron at the beginning (0 min) and the end (30 min) of exposure to a neuronostatin gradient (50 μm in the pipette, top) or control solution (PBS, bottom). The arrows indicate the direction of the gradient. B, upper panel, cumulative distribution of growth cone turning angles for neurons treated with PBS or a gradient of neuronostatin (50 and 500 μm). Lower panel, histograms represent the average turning angles during the 30-min assay for all neurons examined. Numbers of neurons tested are indicated in parentheses. Asterisks indicate data significantly different from the control (p < 0.01). Error bars indicate S.E.