Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Nov 14;283(46):31719–31725. doi: 10.1074/jbc.M802169200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — Photo-cross-linking reactions with B4M-PpsB and B4M-AcpM. A, Western blot analysis of B4M-PpsB before (163 kDa) and after photo-cross-linking in the presence of biotinyl C-12-KasA (205 kDa). This Western blot was developed with an anti-His alkaline phosphatase-conjugated antibody to detect the unbound and KasA-bound forms of PpsB. B, Western blot analysis of KasA before (44 kDa) and after the photo-cross-linking reaction with B4M-AcpM (60 kDa). To detect the unbound and AcpM bound form of KasA, this Western blot was developed with an anti-biotin alkaline phosphatase-conjugated antibody.