TABLE 1.
ATP hydrolysis activity of hMSH2-hMSH6 missense mutants in the presence and absence of a DNA mismatch In the presence of homoduplex DNA, wild-type kcat/Km is 19.7 × 10–4 m–1·min–1.
|
ATPase (G/T DNA)
|
ATPase (no DNA)
|
|||
|---|---|---|---|---|
| kcat | kcat/Km × 10–4 | kcat | kcat/Km × 10–4 | |
| min–1 | m–1·min–1 | min–1 | m–1·min–1 | |
| Wild type | 24.0 ± 4.2 | 35.8 | 1.6 ± 0.2 | 1.6 |
| S144I | 15.6 ± 1.3 | 36.0 | 1.1 ± 0.1 | 3.6 |
| S285I | 13.0 ± 1.0 | 38.3 | 0.8 ± 0.0 | 2.1 |
| G566R | 2.7 ± 0.2 | 12.6 | 1.2 ± 0.3 | 0.6 |
| D803G | 5.6 ± 0.4 | 9.7 | 0.6 ± 0.0 | 3.5 |
| V878A | 8.1 ± 0.8 | 26.2 | 0.5 ± 0.0 | 0.9 |
| R976H | 5.7 ± 0.5 | 30.8 | 1.3 ± 0.1 | 2.1 |
| H1248D | 1.0 ± 1.0 | 13.0 | 0.3 ± 0.0 | 0.6 |