TABLE 2.
DNA and nucleotide binding properties and nucleotide exchange of hMSH2-hMSH6 mutants In the presence of homoduplex DNA, wild-type KD(G/C) is 48.6 nm.
| hMSH6 mutation | DNA binding, KD(G/T) | ATPγS binding, KD(ATP) | ADP-ATP exchange, t1/2 |
|---|---|---|---|
| nm | μm | s | |
| Wild type | 4.78 ± 0.70 | 0.91 ± 0.20 | 4.2 |
| S144I | 5.65 ± 0.19 | 1.10 ± 0.19 | 2.5 |
| S285I | 4.96 ± 0.07 | 0.37 ± 0.26 | 2.1 |
| G566R | 8.51 ± 1.00 | 4.19 ± 1.21 | 3.2 |
| D803G | 11.59 ± 0.97 | 0.41 ± 0.11 | 1.1 |
| V878A | 5.43 ± 0.52 | 1.27 ± 0.23 | 2.8 |
| R976H | 23.15 ± 2.88 | 0.52 ± 0.13 | 9.5 |
| H1248D | 39.79 ± 1.19 | 0.69 ± 0.23 | 4.9 |