TABLE 4.
Mutations resulting from G·U error-prone MMR in cell lysates
|
Reversion frequency × 10–6
|
||||
|---|---|---|---|---|
| No lysate | Tonsil B-cells | Ramos | HeLa | |
| T→A | <4.0 (0)a | 48 (15) | 16.5 (3) | <4.4 (0) |
| T→G | <4.0 (0) | 48 (15) | 27.5 (5) | 8.7 (2) |
| T→C | 7.9 (2) | 26 (8) | 33 (6) | 13 (3) |
| A→G | 12 (3) | 3.2 (1) | 16.5 (3) | 4.4 (1) |
| A→T | 4 (1) | 13 (4) | 11 (2) | <4.4 (0) |
| G→C | 127 (32) | 181 (56) | 231 (42) | 340 (78) |
| G→T | 79 (20) | 90 (28) | 94 (17) | 113 (26) |
| All mutations | 230 (58) | 409 (127) | 430 (78) | 479 (110) |
| A/T mutations | 24 (6) | 138 (43) | 105 (19) | 26 (6) |
| G/C mutations | 206 (52) | 271 (84) | 325 (59) | 453 (104) |
| A/T to G/C ratio p valueb | <0.00001 | 0.008 | 0.123 | |
a
Numbers in parentheses represent number of revertants
b
p values for the ratio of A/T to G/C mutations were calculated by Fisher Exact Test for the null hypothesis that the ratio of mutations at A/T sites versus mutations at G/C sites resulted from repair in the lysate is equal to the background ratio (no lysate)