TABLE 2.
DNA sequences of miRNA and anti-miRNA-146a (AM-146A) probes used in this study
The 5SRNA probe was derived from the first 21 nucleotides of the 107-nucleotide human 5SRNA (20, 26). AM-146a control (AM-146ac), containing the same nucleotide composition as anti-miRNA-146a, is a scrambled anti-miRNA-146a control oligonucleotide.
RNA species | GenBank™ accession number or designation | DNA sequence |
---|---|---|
5SRNA | NR002758 | 5′-ATACTCTGGTTTCTCTTCAGA-3′ |
miRNA-9 | AJ459704 | 5′-TCTTTGGTTATCTAGCTGTATGA-3′ |
miRNA-132 | AJ459743 | 5′-TAACAGTCTACAGCCATGGTCGT-3′ |
miRNA-146a | EU147785 | 5′-TGAGAACTGAATTCCATGGGTT-3′ |
Anti-miRNA-146a | AM-146a | 5′-AACCCATGGAATTCAGTTCTCA-3′ |
AM-146a control | AM-146ac | 5′-CACATACAAGGGACTTTCTACT-3′ |