Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Nov 14;283(46):31371–31377. doi: 10.1074/jbc.M802814200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 1. — Expression and purification of proIL-18. A, vector map of pET28a::IL-18. ProIL-18 was cloned at the NdeI-EcoRI sites. The N-terminal 6-histidine tag and thrombin cleavage site are indicated. B, Coomassie Blue staining of Ni²⁺ purification of His₆-proIL-18 after isopropyl β-d-thiogalactopyranoside induction. Lane 1, sample added to Ni²⁺ column; lane 2, flow-through fraction; lanes 3–14, eluate fractions. C, silver staining of purified proIL-18 before and after thrombin cleavage. Lane 1, His₆-proIL-18; lane 2, thrombin-cleaved proIL-18; lanes 3–8, eluate fractions after Ni²⁺ purification.