FIGURE 5.

Binding of gp5/trx complexes to g4. gp4 is immobilized on the surface of the CM5 chip via amine coupling, and gp5/trx variants are flowed over the surface. 1000 response units of gp4 were coupled to the chip. Binding studies were conducted as described under “Experimental Procedures.” The concentration of gp5 variants in the flow buffer is 0.2 μm and that of trx and trx-K36E is 8 μm (ratio 1:40). A control cell (no coupled gp4) is used as background to subtract for the RU resulting from any nonspecific interaction and bulk refractive index. Red indicates complexes with trx-K36E, and blue indicates complexes with trx-wt.