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A, oxygen binding rate constants for C. aestuarii Hc at pH 5.6 to 8.7. Acetate, MES, and Tris-HCl buffers (50 mm), were used for measurements at pH 5.6, 6.0-6.5, and 7.1-8.7, respectively. B, plots of kobs versus [O2] for the reaction of Hc with oxygen at pH 8.3 (black) and 6.5 (red), together with the least-squares-fitted line according to Equation 1. Experimental conditions are the same as those listed in Fig. 2.
