FIGURE 8.

The TREX1^R114H/R114H and TREX1^WT/R114H enzymes do not inhibit the TREX1M^WT dsDNA degradation activity. Exonuclease reactions (20 μl) were prepared containing nicked dsDNA plasmid 1 (10 μg/ml = 1.6 nm nicks) and no enzyme (lane 2), the indicated concentration of TREX1^WT only (lane 3), or a mixture of TREX1^WT with the indicated increased concentrations of TREX1^R114H/R114H (lanes 4–8), and TREX1^WT/R114H (lanes 9–13). The reactions were 30 min, and the products were subjected to electrophoresis on agarose gels. Lane 1 contains the supercoiled dsDNA plasmid 1. The positions of migration of Form I supercoiled dsDNA (dsDNA), Form II nicked dsDNA (Nicked dsDNA), and circular ssDNA (ssDNA) are indicated.