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. 2008 Nov 11;6(11):e272. doi: 10.1371/journal.pbio.0060272

Figure 2. Cdk5 Gain- or Loss-of-Function Does Not Affect Differentiation Capacity of Adult NPCs.

Figure 2

(A–C) Stable transduced cell lines expressing a control virus (A), cdk5 (B), or DNcdk5 (C) were treated for 4 d with retinoic acid and forskolin to induce neuronal differentiation. Shown are single-channel images for GFP (left panels), MAP2ab (middle panels), and merged images (including DAPI, right panels). Arrows point toward MAP2ab-expressing neuronal cells. There were no significant differences between the groups upon neuronal induction (see Figure 1F).

(D–F) Cocultures of NPCs with hippocampal astrocytes induced neuronal differentiation, but no substantial morphological differences were found after 12 d of coculture between control (D), cdk5-expressing (E), and DNcdk5-expressing (F) cells.

The scale bar in (C), which applies to (A–C), and the scale bar in (F), which applies to (D–F), represent 50 μm.