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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Mol Microbiol. 2008 Sep 30;70(5):1136–1151. doi: 10.1111/j.1365-2958.2008.06462.x

Figure 6. Interactions between AlgQ or Rsd and σ70 region 2 contribute to the regulation of pyocyanin production in P. aeruginosa.

Figure 6

Cultures of plasmid-containing cells were grown in duplicate to an OD600 of 1.5. RNA was isolated and qRT-PCR was used to quantify the abundance of the phzA1 transcript. Transcript abundance was normalized to clpX, the expression of which is not influenced by AlgQ or Rsd. Error bars represent the relative expression values calculated from plus or minus one standard deviation from the mean ΔΔCt.

A. Effect of AlgQ and Rsd on expression of the phzA1 gene. The abundance of the phzA1 transcript was measured in cells of P. aeruginosa strain PAO1 ΔalgQ containing plasmids directing the synthesis of the indicated proteins.

B. Effect of AlgQ and Rsd on expression of the phzA1 gene in a strain synthesizing σ70 [I392F]. The abundance of the phzA1 transcript was measured in cells of P. aeruginosa strain PAO1 ΔalgQ σ70 [I392F] containing plasmids directing the synthesis of the indicated proteins.

C. Effect of Rsd mutants on expression of phzA1. The abundance of the phzA1 transcript was measured in cells of P. aeruginosa strain PAO1 ΔalgQ containing plasmids directing the synthesis of the indicated proteins.