Figure 1.

Mapping of PIAS1- and Stat1-interacting domains. (A) Identification of the Stat1-interacting domain of PIAS1. Expression vectors encoding full-length PIAS1 or various PIAS1 deletions fused to VP16 activation domain together with Stat1β expression construct were transformed into yeast EGY48-pJK103 cells. (B) Identification of the PIAS1-interacting domain of Stat1. Expression vectors encoding full-length Stat1 or various Stat1 deletions fused to the LexA DNA-binding domain together with a construct expressing the COOH terminus of PIAS1 (PIAS1c) were transformed into yeast EGY48-pJK103 cells. (+) Presence of blue yeast cells; (−) no blue yeast cells. (Zn²⁺) putative zinc-binding motif; (CCD) coil-coil domain; (DBD) DNA-binding domain; (LD) linker domain; and (SH2) Src homology 2 domain.