Table 1.

The predicted splicing parameters and RPA results

	Exon/intron/exon combination
	53/53/54	56/56/57	60/60/61	63/63/64
CVa	0.90, 0.60	0.84, 0.97	0.94, 0.98	0.95, 0.90
ΔDGo37b	−7.7	−10.4	−11.7	−10.6
Total RNA	68.7 ± 27.8	2.4 ± 1.2c	342.2 ± 24.3d	159.9 ± 9.4c
Unspliced RNA	20.0 ± 9.1	1.6 ± 1.2	87.1 ± 11.2d	20.4 ± 9.1
Spliced RNA	48.8 ± 20.6	0.8 ± 0.2	255.1 ± 20.6d	139.5 ± 6.4d
S/U ratioe	2.5 ± 0.3	1.0 ± 0.6	3.0 ± 0.4	7.1 ± 0.9d
% of splicing	71.1	31.3c	74.5	87.2c

^aConsensus splicing values (CV) at the 5′ and the 3′ ends of each indicated intron (from ref. 18). A higher value indicates a better match.

^bFree energy exchange during U1snRNA annealing (Kcal/mole). The more negative the value, the more favorable the annealing.

^cValues are statistically different from each other in the same category (P < 0.014).

^dValues are statistically different from other exon/intron/exon combinations in the same category (P < 0.016).

^eThe ratio of spliced (S) to unspliced (U) RNA transcript.