Table 2.

Primers, annealing temperatures and enzymes used in the genotyping assays for each of the candidate single nucleotide polymorphisms (SNPs)

Gene	Reference SNP number (rs#)	Forward primer Reverse primer	Annealing temp (°C)	Enzyme
MT-RNR1	—	5′ GGGTCGAAGGTGGATTTAGC 3′	54	BsmAI
		3′ ACTCTGGTTCGTCCAAGTGC 5′
MYO6	rs13211391	5′ GGGAGCAAGCTTTATTCGTT 3′	49	SspI
		3′ CTATGTTGCCCAGGCTGACT 5′
MYO7A	rs948962	5′ TCTTTCCTGAGAAGGAGCAG 3′	57	EcoO109I
		3′ ATGGGCCGAGCTTTCTTTAT 5′
	rs1052030	5′ CTTCTCTTCCCCCTTGTGTG 3′	51	DpnII
		3′ CAGAGTCGCAGAGCTTCACC 5′
MYO15	rs854777	5′ CACTCCCCAACCTGACATCT 3′	57	SfoI
		3′ GCTCAGCTCCTAGAGGGACA 5′
BDNF	rs6265	5′ GAGGCTTGACATCATTGGCT 3′	60	Eco72I
		3′ CGTGTACAAGTCTGCGTCCT 5′
NOS3	rs1799983	5′GACCCTGGAGATGAAGGCAGGAG3′	60	BanII
		3′ACCTCCAGGATGTTCTAGCGGTGA5′
	rs10952298	5′ CCAGGCCCACCCCAACCTTAT 3′	53	MspI
		3′ TCATTCAGTGACGCACGCTT 5′
	Intron 4	5′ CCTGGTTATCAGGCCCTATG 3′	59	N/A
	VNTR	3′ AGGCTGCTCCTGCTACTGAC 5′
GSTP1	rs1695	5′CTCTATGGGAAGGACCAGCAGGA3′	65	Alw26I
		3′ CAAGCCACCTGAGGGGTAAGG 5′
	rs1138272	5′ TTGACAGGATTTGGTACTAGCC 3′	52	AciI
		3′ TGGTCTCCCACAATGAAGGT 5′
GSTM3	rs1799735	5′ CCTCAGTACTTGGAAGAGCT 3′	52	MnlI
		3′ CACATGAAAGCCTTCAGGTT 5′
GSTZ1	rs7972	5′ TGACCACCCAGAAGTGGTAG 3′	52	FokI
		3′ AGTCCACAAGACACAGGTTC 5′
	rs3177427	5′ TGACCACCCAGAAGTGGTAG 3′	52	Alw26I
		3′ AGTCCACAAGACACAGGTTC 5′