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. 2008 Nov 18;3(11):e3726. doi: 10.1371/journal.pone.0003726

Figure 2. Verification of the miRNA microarray results by qRT-PCR.

Figure 2

QRT-PCR results of miRNA expression level in undifferentiated HES1 and HES2 cells or cells differentiated with NaB for 7d or 14d. Results are expressed as relative quantification of the miRNA expression level in each sample relative to undifferentiated cells of the respective line, and normalized to RNU43. Each reaction was performed in triplicate and the average ct was used for RQ calculation. RQ–Relative Quantification; Con–control cells grown with the same media as treated cells, but without NaB; NaB–0.5 mM Na Butyrate; F–Feeder, mitotically arrested foreskin cells (feeder cells of hESC); EC–Embryonal Carcinoma 2102Ep cell line; EL–human embryonic liver of stage 7w from gestation; AL–human normal adult liver; NS–Neurospheres, HES1 cells differentiated for 4.5w towards neural fate.