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. Author manuscript; available in PMC: 2008 Nov 10.
Published in final edited form as: Cell Cycle. 2008 Mar 18;7(10):1490–1495. doi: 10.4161/cc.7.10.5963

Figure 2.

Figure 2

Activation of ATM and phosphorylation of histone H2AX in A549 cells treated with H2O2. The cells were untreated (time 0) or treated in cultures with 200 μM H2O2 for time periods as shown in the respective panels. Bivariate distributions (DNA content vs ATM-S1981P, or vs γH2AX IF) represent the IF of these phospho-proteins integrated over the nucleus with respect to cell cycle phase. The dashed skewed lines show the upper threshold level of ATM-S1981P or γH2AX IF for 97% of interphase (G1 + S) cells in the untreated (time 0) cultures. The DNA content histograms in the bottom panels show the cell cycle distribution at time 0 and after 4 h of treatment with H2O2. A minor accumulation of very early S-phase cells is apparent after 4 h of treatment with H2O2 (arrow).