Figure 1.

Inducible deletion of Cnb1 in bone marrow-derived lineages. (A) Expression of components of the calcineurin/NFAT pathway was analysed by RT–PCR on sorted cell populations. (B) Cnb1 conditional knockout Mx-1^Cre mice were treated with poly I:C to induce the expression of Cre; whole or sorted bone marrow fractions from Cnb1-deficient and control donors were transplanted into irradiated CD45.1⁺ B6.SJL congenic recipients alone or in conjunction with CD45.1⁺ helper B6.SJL bone marrow. (C) PCR of genomic DNA from tail or Cnb1-deficient and control bone marrow after treatment with poly I:C. BM, bone marrow; NFAT, nuclear factor activating T-cells; poly I:C, poly inosilic acid; RT–PCR, reverse transcription PCR.