Fig. 2.
The GDNF-vector or the BDNF-vector supported long-term expression of GDNF or BDNF, respectively, in striatal neurons. The rats were sacrificed at 7 months after gene transfer. (a) GDNF-vector with GDNF-IR, (b) BDNF-vector with BDNF-IR, and (c) no GDNF-IR was detected in the contralateral striatum (GDNF-vector). (d–f) GDNF and BDNF were detected in the same striatal cells in a rat that received a mixture of the GDNF- and BDNF-vectors; (d) GDNF-IR, (e) BDNF-IR, and (f) merge. (g–l) GDNF or BDNF were expressed in neurons in rats that received either the GDNF-vector or the BDNF-vector, respectively. (g–i) GDNF-vector; (g) GDNF-IR, (h) NeuN-IR, and (i) merge. Arrows, costained cells; arrowheads, cells that contain only NeuN-IR. (j –l) BDNF-vector; (j) BDNF-IR, (k) NeuN-IR, and (l) merge. Scale bars: (a–c) 40 μm; (d–l) 50 μm.