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Defective VAMP2 endocytosis does not affect neuron morphology. Live neurons coexpressing EGFP (green) and wild-type VAMP2-BTX or mutant VAMP2M46A-BTX were labeled with Alexa555-BTX (red) at 4°C and then fixed. Although the mutant VAMPM46A-BTX is mislocated to all neurites (arrowheads), the morphology of the neuron is not changed. Scale bar, 10 μm.
