Figure 3.

Effects of AP180siRNA or CALMsiRNA on axon and dendrite outgrowth in younger and older neurons. A, B, Quantification of axonal or dendritic length as a function of culture and transfection. n = 15–45 neurons. Data represent means ± SEM. DIV, days in vitro. C, Hippocampal neurons (10 div) expressing AP180siRNA or CALMsiRNA. Scale bar, 10 μm. Additional examples are shown in supplemental Figure S5B, available at www.jneurosci.org as supplemental material. D, Sholl analysis of the neurons shown in C. n = 20 neurons in all groups. Data represent means ± SEM.