Figure 4.

Overexpression of AP180 and CALM induces the hippocampal neurons to grow multiple axons. A, Immunolabeling of the axonal marker tau1 (red) in neurons coexpressing EGFP (green) and AP180 or CALM as indicated. Arrowheads indicate tau1-labeled axons. Scale = 10 μm. B, Quantification of the neurons that have two or more tau1-labeled axons. *p < 0.01; **p < 0.001; n = 45 neurons. C, The mean length of the axons in neurons overexpressing AP180 or CALM. n = 45 neurons. Data represent means ± SEM in B, C.