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Knockdown of CALM but not AP180 disrupts forward transport. A, Confocal images showing total VSVG-GFP (green) and surface VSVG (red) in the control and AP180siRNA neurons (16 div) or CALMsiRNA neurons (16 div). Arrowheads indicate surface VSVG clusters. Scale bar, 10 μm. B, Quantification of surface to total ratio of VSVG in the control and the siRNA neurons. **p < 0.001, n = 20 neurons in all groups.
