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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: J Biomed Opt. 2008;13(3):031207. doi: 10.1117/1.2940366

Fig. 2.

Fig. 2

Bleed-through control values determined by linear regression. (a) The amounts of background-subtracted fluorescence from the donor-labeled ERα (CFP or mCFP) in the FRET or acceptor channel in relationship to the donor channel. The r2 values indicate whether the data points fall along a straight line. For a linear relationship, the slope of the line represents the bleed-through contributions of the donor to each channel. (b) Similar analysis for the acceptor-labeled ERα (YFP or mYFP). The bleed-through and r2 values for each of the five experiments are shown in Table 2.