Table 2.

Contributions of acceptor or donor to each collection channel determined from control cells for experimental studies in Figs. 4 and 5, calibration studies in Fig. 3. (A low r² value and zero slope indicates zero contributions of the fluorescent protein to that channel. Ratios established from plots with high r² values represent fluorescent contributions to the channels relative to that of the fluorescent protein in its cognate channel.)

	ER Experiment 1	ER Experiment 2	ER Experiment 3	ER Experiment 4	ER Experiment 5	Mean ± SD ER studies	Mean ± SD AR studies
	Ratio (r2)	Ratio (r2)	Ratio (r2)	Ratio (r2)	Ratio (r2)	Ratio (r2)	Ratio (r2)
Donor (ER-CFP)
to FRET channel	0.579 (0.997)	0.572 (0.996)	0.577 (0.996)	0.558 (0.997)	0.563 (0.997)	0.570 ± 0.009 (0.997 ± 0.001)	0.563 ± 0.011 (0.988 ± 0.009)
to Acceptor channel	0.001 (0.043)	0.001 (0.048)	0.001 (0.113)	0.000 (0.037)	0.001 (0.072)	0.001 ± 0.000 (0.062 ± 0.031)	0.002 ± 0.001 (0.096 ± 0.070)
Acceptor (ER-YFP)
to FRET channel	0.132 (0.992)	0.133 (0.992)	0.130 (0.983)	0.130 (0.990)	0.132 (0.989)	0.131 ± 0.001 (0.989 ± 0.004)	0.126 ± 0.009 (0.956 ± 0.043)
to Donor channel	0.001 (0.039)	0.003 (0.080)	0.002 (0.022)	0.002 (0.037)	0.001 (0.020)	0.002 ± 0.001 (0.040 ± 0.024)	−0.001 ± 0.002 (0.025 ± 0.035)
Donor (ER- mCFP)
to FRET channel	0.584 (0.996)	0.569 (0.997)	0.582 (0.998)	0.562 (0.996)	0.579 (0.998)	0.575 ± 0.009 (0.997 ± 0.001)
to Acceptor channel	0.001 (0.157)	0.001 (0.059)	0.001 (0.124)	0.000 (0.064)	0.001 (0.157)	0.001 ± 0.000 (0.112 ± 0.048)
Acceptor (ER- mYFP)
to FRET channel	0.127 (0.979)	0.127 (0.992)	0.129 (0.994)	0.127 (0.991)	0.128 (0.989)	0.128 ± 0.001 (0.989 ± 0.006)
to Donor channel	0.001 (0.006)	0.002 (0.047)	0.002 (0.044)	0.001 (0.010)	0.002 (0.031)	0.001 ± 0.001 (0.027 ± 0.019)