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. 2000 Apr 25;97(10):5279–5284. doi: 10.1073/pnas.080409297

Figure 2.

Figure 2

Expression of p57kip2 in cultured cells with or without IGF-II. (A) Poly(A)+ RNA was extracted from confluent (Conf) or exponentially growing (Exp) cells cultivated in the presence of 10% (vol/vol) FCS (high serum) or from cells that had been serum starved for 24 h (low serum) in the presence of different concentrations of IGF-II. mRNA (0.5 μg) was analyzed by Northern analysis with a p57kip2 probe. (B) Western blot analyses with p57KIP2 antibody. The blot containing protein extracts from PEFs treated with 0, 10, or 50 ng of IGF-II was probed with anti-p57KIP2 and with anti-α-tubulin as a loading control. The data shown are representative of three independent experiments. (C) p57kip2 mRNA expression in cells cultivated with 10% (vol/vol) (high serum) or 0.5% (vol/vol) (low serum) FCS. Data represent the p57kip2 signal normalized with the Gapdh control signals. (D) The percentage of inhibition of p57kip2 expression corresponds to the ratio of the p57kip2 signal normalized with the Gapdh control signals between the IGF-II-treated and untreated cells.