Figure 4.

Down-regulation of the expression of p57^kip2 gene in T^hp embryos. (A) Northern blot analysis of total RNA isolated from normal (N) and mutant embryos (T^hp) at different stages of gestation hybridized with p57^kip2. Gapdh expression is used as a control. (B) Graphic representation shows the average of the p57^kip2 activity for five embryos normalized to Gapdh expression, compared with p57^kip2 expression of normal embryos from the same litter. Error bars represent the standard errors. Statistically significant differences are indicated by an asterisk (Student's t test; *, P < 0.05). (C) Western blot analyses with p57^KIP2 antibody. The blot containing mutant (T^hp) and normal (N) embryo protein extracts at e14.5 was probed with anti-p57^KIP2 and with anti-α-tubulin as a loading control.