Figure 3.

Effect of an increased serum/cell ratio on antibody uptake. Y-axes and antibodies are the same as in previous figures. In the initial conditions, antibodies are supposed to have a concentration equal to half of the amount necessary to give a ‘+’ in the antiglobulin test. A serum/cell ratio of 40 corresponds to two volumes of serum/plasma for one volume of 5% red cells. Higher ratios are obtained by adding more serum/plasma. Anti-K is greatly enhanced. In contrast, the uptake of low affinity antibodies (anti-D and anti-c) is only modestly increased.