Table VI.
Instructions to calculate the number of antibody molecules bound per cell, when the equilibrium constant and the total concentration of the antibody are known
| Cell | Text to be entered |
|---|---|
| A1 | Number of antibody molecules bound per cell, when the equilibrium constant and the total concentration of the antibody are known |
| A3 | Volume of serum/plasma (μL) |
| A4 | Volume of RBC suspension (μL) |
| A5 | [RBC] (%) |
| A6 | Volume of diluent/potentiator (μL) |
| A7 | Antigen sites per cell |
| A8 | Equilibrium constant |
| A9 | Total antibody concentration (mol/L) |
| A10 | Avogadro’s number |
| A12 | Number of RBC per litre |
| A13 | Total antigen concentration (mol/L) |
| A14 | First solution |
| A15 | Second solution |
| A16 | Antigen-antibody complex concentration (mol/L) |
| A17 | Free antibody concentration (mol/L) |
| A18 | Bound antibody (%) |
| A19 | Number of antibody molecules bound per cell |
|
| |
| Value to be entered | |
|
| |
| B3 | The volume of serum/plasma in the test system (μL), e.g. 25 |
| B4 | The volume of the RBC suspension (μL), e.g. 50 |
| B5 | The concentration of the RBC suspension (%), e.g. 0.8 |
| B6 | The volume of the diluent/potentiator (if not included in the RBC suspension) (μL), e.g. 0 |
| B7 | The number of antigen sites per cell, e.g. 12,000 |
| B8 | The equilibrium constant, e.g. 2E8 |
| B9 | The total antibody concentration (mol/L), e.g. 6.63E-11 |
| B10 | Avogadro’s number: 6.022E23 |
|
| |
| Formula to be entered | |
|
| |
| B12 | =B5/100*B4/(B3+B4+B6)* POWER (10,13) |
| B13 | =B7*B12/B10 |
| B14 | =(B13+B9+1/B8)/2+ POWER (POWER ((B13+B9+1/B8)/2,2)-B13*B9,0.5) |
| B15 | =(B13+B9+1/B8)/2- POWER (POWER ((B13+B9+1/B8)/2,2)-B13*B9,0.5) |
| B16 | =IF(OR(B14>B9,B14<0),B15,B14) |
| B17 | =B9-B16 |
| B18 | =B16/B9*100 |
| B19 | =B16*B10/B12 |