Figure 2. Sustained canonical NF-κB activation induces a cell cycle effector suppression response in conjunction with the activation of known NF-κB targets.

The expression of twenty-one genes (six induced and 15 repressed) are examined by real time PCR in MEFs stably expressing a constitutively activated IKKβ mutant in a puromcyin resistant retroviral vector (IKKβca-BIP) vs. MEFs harboring a BIP empty retroviral vector. All RNAs were prepared from populations of MEFs 7 days post-infection (dpi) with either IKKβca-BIP or empty BIP. Fold change values for 7 dpi IKKβca-BIP vs. 7 dpi BIP MEFs were derived by the ΔΔCt method (with duplicate samples differing by no more than 0.5Ct) as described in Materials and Methods (Dussault and Pouliot, 2006). Relative fold change values were the averages of duplicate samples from one representative experiment out of two independent experiments producing similar results.