Skip to main content
. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Cell Physiol. 2009 Jan;218(1):215–227. doi: 10.1002/jcp.21596

Figure 3. Gene repression induced by IKKβca requires canonical NF-κB signaling.

Figure 3

The canonical NF-κB requirement for gene repression by IKKβca were evaluated in a population of MEFs stably expressing an IκBα super-repressor (SR) (IBIN-MEFs). Fold change values of four induced and six repressed genes were determine by real time PCR on total cell RNAs prepared from 7 dpi IKKβca-BIP in IBIN-MEFs vs. 7 dpi IKKβca-BIP in wild type MEFs. Results were quantified as described in Materials and Methods and in Figure 2's legend and are averages of duplicate samples from one representative experiment out of two independent experiments producing similar results.