Figure 5. Enforced IKKβca expression in immortalized MEFs produces a G1/S cell cycle block during the 1^st week of culture.

Flow cytometry was performed on propidium iodide (PI) stained cells to reveal their cell cycle distribution profiles (as described in Materials and Methods). Data was analyzed with FACSDiva software (Becton-Dickinson). The percentages of cells in G1, S and G2 phases are indicated. Results are representative of several independent experiments. Images of 7 dpi IKKβca-BIP and 7 dpi Wt. MEF-BIP control cells (200X magnification phase contrast microscopy) are shown next to each FACS profile.