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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Cell Physiol. 2009 Jan;218(1):215–227. doi: 10.1002/jcp.21596

Figure 8. NF-κB mediated gene repression is an active mechanism involving histone deacetylases.

Figure 8

A: HDAC inhibition prevents IKKβca induced gene repression. 7 dpi IKKβca MEFs were treated with 100 nM trichostatin A (TSA) for 4 hours. The expressions of 5 induced and 6 repressed target gene are shown as fold change values in 7 dpi IKKβca MEFs +TSA vs. 7 dpi BIP control MEFs. B: HDAC inhibition or constitutive expression of IκBαSR blocks TNFα induced gene repression. Expression results for four repressed genes (FoxM1, Ect2, Casp8 and Brca2) that were identified by our microarray screens are shown as fold change values in Wt MEFs (+TNFα), IBIN-MEFs and Wt. MEFs (+TNFα & TSA) vs. Wt. unstimulated MEFs in each case as indicated. For the TSA experiment wild type MEFs were pretreated with 100 nM Trichostatin-A (TSA) for two hours followed by TNFα + TSA for an additional two hours. Results were quantified as described in Materials and Methods and also in Fig. 2 legend and are expressed as averages of duplicate samples from one representative experiment out of two independent experiments producing similar results.