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. Author manuscript; available in PMC: 2009 Oct 17.
Published in final edited form as: Cell. 2008 Oct 17;135(2):250–260. doi: 10.1016/j.cell.2008.09.054

Figure 1. HerA and NurA degrade dsDNA cooperatively with Mre11/Rad50 in magnesium.

Figure 1

(A) Nuclease assays were performed using 2.5 kb internally [32P]-labeled dsDNA. Reactions contained 2.7 nM wild-type HerA and 19.2 nM wild-type NurA, 5 mM MgCl2, 1 mM ATP, and 100 mM NaCl. Wild-type MR complex was included in the reaction at 0.3, 3.3, 33, and 330 nM. HerA molar concentrations are given as a hexamer of 370 kDa, NurA concentrations are given as a monomer of 52 kDa, and MR complex is given as a 2:2 stoichiometric complex of 306 kDa.

(B) Reactions performed as in (A) with HerA wild-type or K164A, NurA wild-type or D51A, and 3.3 nM MR wild-type, M(H85L)R, or MR(S793R) as indicated in 80 mM NaCl.